Frykberg L, Palmieri S, Beug H, Graf T, Hayman M J, Vennström B
Cell. 1983 Jan;32(1):227-38. doi: 10.1016/0092-8674(83)90513-5.
Mutants of avian erythroblastosis virus (AEV) were constructed by deleting large nucleotide segments in each of the viral oncogenes termed v-erbA and v-erbB. Mutants in erbA (erbA -B +) retained the ability to transform fibroblasts in vitro, and these cells exhibited most of the transformation characteristics that typify wild-type AEV-transformed fibroblasts. In addition, the mutants induced small erythroid colonies upon infection of bone marrow cells in culture. Chickens inoculated with erbA -B + virus or with erbA -B +-transformed cells developed sarcomas or atypical erythroid leukemias. The erythroid cells transformed in vivo or in vitro by the erbA -B + viruses appeared not to be as tightly blocked in differentiation as wild-type transformed cells. In contrast, fibroblasts infected with the erbA +B - mutant resembled normal cells in all transformation parameters tested, and no bone marrow cell transformation was observed with the mutant. The results indicate that the main transforming properties of AEV are encoded in erbB and that its effects are enhanced by erbA.
通过删除称为v-erbA和v-erbB的每个病毒癌基因中的大片段核苷酸，构建了禽成红细胞增多症病毒（AEV）的突变体。erbA中的突变体（erbA -B +）保留了在体外转化成纤维细胞的能力，并且这些细胞表现出大多数典型的野生型AEV转化成纤维细胞的转化特征。此外，这些突变体在感染培养中的骨髓细胞时诱导产生小的红系集落。接种erbA -B +病毒或erbA -B +转化细胞的鸡会发生肉瘤或非典型红系白血病。erbA -B +病毒在体内或体外转化的红系细胞似乎在分化上不像野生型转化细胞那样受到严格的阻滞。相比之下，感染erbA +B -突变体的成纤维细胞在所有测试的转化参数方面都类似于正常细胞，并且该突变体未观察到骨髓细胞转化。结果表明，AEV的主要转化特性由erbB编码，并且其作用由erbA增强。
