Hobden A N, Cundliffe E
Biochem J. 1978 Jan 15;170(1):57-61. doi: 10.1042/bj1700057.
A new technique was developed for measuring the amount of peptidyl-tRNA in a protein-synthesizing system in vitro. By this technique the course of the puromycin reaction may be followed and the modes of action of various inhibitors of protein synthesis readily determined. We conclude that the polypeptide alpha sarcin inhibits the binding of aminoacyl-tRNA into the ribosomal 'A' site, that sparsomycin inhibits the peptidyl transferase reaction and that cycloheximide may block translocation.
一种用于体外测量蛋白质合成系统中肽基 - tRNA含量的新技术被开发出来。通过这种技术，可以追踪嘌呤霉素反应的进程，并能轻松确定各种蛋白质合成抑制剂的作用模式。我们得出结论：α - 肌动蛋白多肽抑制氨酰 - tRNA与核糖体“A”位点的结合，稀疏霉素抑制肽基转移酶反应，而环己酰亚胺可能会阻断转位。
