Dependence on pH of substrate binding to lactose carrier in Escherichia coli cytoplasmic membranes.
作者信息
Yamato I, Rosenbusch J P
出版信息
FEBS Lett. 1983 Jan 10;151(1):102-4. doi: 10.1016/0014-5793(83)80352-4.
PMID:6297984
Abstract
Lactose permease in Escherichia mediates proton-substrate cotransport. The molecular mechanism of this process is not understood. We examined the effect of proton concentration on the binding of a substance analogue to the carrier. The dissociation constant of p-nitrophenyl-alpha-galactoside from the carrier was dependent on pH, with an apparent pKa of 9.7.
摘要
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