Colquhoun J E, Dresden M H
Dev Biol. 1983 Jan;95(1):193-9. doi: 10.1016/0012-1606(83)90017-9.
Prolyl hydroxylase activity was measured to obtain insight into changes in collagen metabolism during forelimb regeneration in the adult newt, Notophthalmus viridescens. Activity increased markedly during the redifferentiative stages, remained elevated during digit formation, and decreased as regeneration neared completion. The greatest activity, seen during early digit formation, represents a 20-fold increase in activity over the level seen in nonregenerating limbs. The profile of enzyme activity correlates with the rate of collagen synthesis and the soluble collagen content of the regenerating tissue. Enzyme extracted from limbs in the early stages of regeneration can be activated in vitro upon preincubation with cofactors; however, preincubation has little, if any, effect on enzyme extracted from limbs at the later digit-forming stages.
为深入了解成年绿螈（Notophthalmus viridescens）前肢再生过程中胶原蛋白代谢的变化，我们对脯氨酰羟化酶活性进行了测定。在再分化阶段，该酶活性显著增加，在指形成过程中保持升高，而随着再生接近完成，活性降低。在早期指形成阶段观察到的最大活性，相较于未再生肢体中的水平，活性增加了20倍。酶活性曲线与再生组织中胶原蛋白的合成速率和可溶性胶原蛋白含量相关。从再生早期阶段的肢体中提取的酶，在与辅因子预孵育后可在体外被激活；然而，预孵育对从后期指形成阶段的肢体中提取的酶几乎没有影响（若有影响也微乎其微）。
