Wang Z, Roberts J M, Grant P G, Colman R W, Schreiber A D
Thromb Haemost. 1982 Dec 27;48(3):301-6.
We investigated the effect of the Chinese herb Injectio Salvia Miltiorrhizae (ISM) on human platelet function in vitro. ISM inhibited platelet aggregation and serotonin release induced by either ADP or epinephrine in a dose dependent manner. This effect of ISM was observed with both gel-filtered platelets (ID50 = 8-30 micrograms ISM/ml gel-filtered platelets) and platelets in plasma (ID50 = 400-900 micrograms ISM/ml of platelet-rich plasma). The active molecule(s) in ISM was heat stable, resistant to acid, base and proteolysis and fractionated on Sephadex 6-25 at MW approximately 280. ISM did not interact with the platelet alpha-adrenergic receptor, but increased cAMP in intact platelets. The results are consistent with the concept that ISM inhibition of platelet aggregation and release is mediated by an increase in platelet cAMP. The exact mechanism whereby ISM increases platelet cAMP appears to be that of inhibition of cyclic AMP phosphodiesterase. The effect of ISM on platelet function is one mechanism which might explain the therapeutic effect of ISM in experimental and clinical coronary artery disease.
我们研究了中药丹参注射液（ISM）对人血小板功能的体外作用。ISM以剂量依赖性方式抑制由ADP或肾上腺素诱导的血小板聚集和5-羟色胺释放。在凝胶过滤血小板（ID50 = 8 - 30微克ISM/毫升凝胶过滤血小板）和血浆中的血小板（ID50 = 400 - 900微克ISM/毫升富血小板血浆）中均观察到ISM的这种作用。ISM中的活性分子耐热、耐酸、耐碱且抗蛋白水解，在Sephadex 6 - 25上分级分离，分子量约为280。ISM不与血小板α-肾上腺素能受体相互作用，但能增加完整血小板中的cAMP。这些结果与ISM对血小板聚集和释放的抑制作用是由血小板cAMP增加介导的这一概念一致。ISM增加血小板cAMP的确切机制似乎是抑制环磷酸腺苷磷酸二酯酶。ISM对血小板功能的影响是一种可能解释ISM在实验性和临床冠状动脉疾病中治疗作用的机制。
