Sorensen M B, Butler L G
Arch Biochem Biophys. 1983 Jan;220(1):225-31. doi: 10.1016/0003-9861(83)90404-6.
Bovine intestinal 5'-nucleotidase has been partially purified and characterized for comparison with two other phosphohydrolases from the same tissue, alkaline phosphatase and 5'-nucleotide phosphodiesterase, which are closely related structurally and mechanistically. Kinetic studies with a variety of nucleotides and phosphonate analogs show that, although 5'-nucleotidase is a monoesterase like alkaline phosphatase, it more closely resembles 5'-nucleotide phosphodiesterase in its high affinity and specificity for nucleotide binding. 5'-Nucleotidase is bound very strongly by an affinity column containing a bound phosphonate analog of ADP but is not bound by an affinity column containing a non nucleotide phosphonate which selectively binds alkaline phosphatase. 5'-Nucleotidase is strongly bound by immobilized antibodies prepared against 5'-nucleotide phosphodiesterase, and is less strongly bound by immobilized antibodies prepared against alkaline phosphatase. We conclude that 5'-nucleotidase is structurally more similar to 5'-nucleotide phosphodiesterase than to another monoesterase, alkaline phosphatase.
牛小肠5'-核苷酸酶已被部分纯化并进行了特性分析，以便与来自同一组织的其他两种磷酸水解酶——碱性磷酸酶和5'-核苷酸磷酸二酯酶进行比较，这两种酶在结构和机制上密切相关。对多种核苷酸和膦酸酯类似物的动力学研究表明，尽管5'-核苷酸酶是一种像碱性磷酸酶一样的单酯酶，但它在对核苷酸结合的高亲和力和特异性方面更类似于5'-核苷酸磷酸二酯酶。5'-核苷酸酶被含有结合了ADP膦酸酯类似物的亲和柱强烈结合，但不被含有选择性结合碱性磷酸酶的非核苷酸膦酸酯的亲和柱结合。5'-核苷酸酶被针对5'-核苷酸磷酸二酯酶制备的固定化抗体强烈结合，而被针对碱性磷酸酶制备的固定化抗体结合得较弱。我们得出结论，5'-核苷酸酶在结构上与5'-核苷酸磷酸二酯酶的相似性高于与另一种单酯酶碱性磷酸酶的相似性。
