Necessary P C, Ebner K E
Biochem Biophys Res Commun. 1983 Feb 28;111(1):224-30. doi: 10.1016/s0006-291x(83)80140-5.
Ovine prolactin specifically bound to rabbit mammary membrane prolactin receptor was rapidly dissociated in a pH dependent manner with 0.1M ammonium acetate. Up to 75% of the bound hormone was dissociated at pH 4.0 or lower in less than 5 minutes. The pK of the dissociation was 4.7, implicating one or more critical carboxyl groups. Exposure of the membrane bound receptor to the dissociating buffer for up to one hour did not reduce its ability to bind hormone. The dissociated hormone was characterized as intact ovine prolactin by Bio-Gel P-150 gel chromatography and by its ability to bind to fresh rabbit prolactin receptor with the same binding affinity as native hormone.
绵羊催乳素与兔乳腺膜催乳素受体的特异性结合，在pH值依赖的情况下，可被0.1M醋酸铵快速解离。在pH 4.0或更低时，高达75%的结合激素在不到5分钟内解离。解离的pK为4.7，这表明存在一个或多个关键羧基。将膜结合受体暴露于解离缓冲液中长达一小时，并未降低其结合激素的能力。通过Bio-Gel P-150凝胶过滤法以及解离激素与新鲜兔催乳素受体结合的能力（与天然激素具有相同的结合亲和力），可将解离的激素鉴定为完整的绵羊催乳素。
