Maderspach K, Fajszi C
Brain Res. 1983 Feb;282(3):251-7. doi: 10.1016/0165-3806(83)90064-0.
The beta-receptors of intact neuronal and glial cells of chick embryonic brain were studied via the specific binding of the beta-antagonist [3H]dihydro-L-alprenolol ( [3H]DHA). Cells were cultivated in either highly homogeneous or mixed populations; the neuronal cells were also grown under the influence of glial conditioned medium (GCM) or 10(-11)-10(-10) M L-norepinephrine or L-isoproterenol. The beta-receptors of both neuronal and glial cells proved to be positively cooperative (n = 2.5) and of high affinity, with a Kdapp of 98 and 44 pM, respectively. The Kdapp value was influenced only slightly by the different culture conditions. The receptor concentration was relatively low in the homogeneous neuronal and glial cultures (Bmax = 6.4 and 3.3 fmol/10(6) cells, respectively). It increased by a factor of 2-3 if development of the neuron-glia contacts in the culture was possible (mixed cultures). GCM and beta-agonists elevated the number of beta-receptors of the neuronal cells approximately 4-fold, even in the absence of glial cells. This receptor-number change was preceded by a well observable morphological differentiation. Both the morphological and the beta-receptor effects of L-norepinephrine were antagonized by L-propranolol. The beta-receptor number increased about 2-fold during a 10-day in vitro development, even in neuron-glia mixed cultures.
通过β拮抗剂[3H]二氢-L-心得安（[3H]DHA）的特异性结合，研究了鸡胚脑完整神经元和胶质细胞的β受体。细胞在高度均匀或混合群体中培养；神经元细胞也在胶质细胞条件培养基（GCM）或10（-11）-10（-10）M L-去甲肾上腺素或L-异丙肾上腺素的影响下生长。神经元和胶质细胞的β受体均表现为正协同作用（n = 2.5）且亲和力高，其表观解离常数（Kdapp）分别为98和44 pM。不同培养条件对Kdapp值的影响很小。在均匀的神经元和胶质细胞培养物中，受体浓度相对较低（Bmax分别为6.4和3.3 fmol/10（6）个细胞）。如果培养物中神经元与胶质细胞的接触有可能发生（混合培养），则受体浓度会增加2至3倍。即使在没有胶质细胞的情况下，GCM和β激动剂也能使神经元细胞的β受体数量增加约4倍。这种受体数量的变化之前会有明显的形态学分化。L-去甲肾上腺素的形态学和β受体效应均被L-普萘洛尔拮抗。即使在神经元-胶质细胞混合培养物中，体外培养10天期间β受体数量也会增加约2倍。
