Ciliberto G, Raugei G, Costanzo F, Dente L, Cortese R
Cell. 1983 Mar;32(3):725-33. doi: 10.1016/0092-8674(83)90058-2.
We have shown that the 34 bp internal control region of the somatic 5S RNA gene from Xenopus borealis can be split into two separable components. A maxigene carrying an insertion between nucleotide 71 and nucleotide 74 of the coding region is actively transcribed in the nucleus of X. laevis oocytes, giving rise to a maxitranscript with initiation and termination points identical with those of the wild-type transcript. The first 11 bases of the 5S RNA gene promoter are shown to be structurally and functionally homologous with the first component (box A) of the promoter for tRNA genes. This was shown by constructing hybrid 5S RNA-tRNAPro and tRNAPro-5S RNA genes that were efficiently transcribed in the X. laevis oocytes. Initiation of transcription appears to be a complex phenomenon in which both components of the internal promoter play a role.
我们已经证明，来自北方爪蟾的体细胞5S RNA基因的34 bp内部控制区可分为两个可分离的组分。一个在编码区核苷酸71和核苷酸74之间带有插入片段的大基因在非洲爪蟾卵母细胞核中被活跃转录，产生一个起始和终止点与野生型转录本相同的大转录本。5S RNA基因启动子的前11个碱基在结构和功能上与tRNA基因启动子的第一组分（A框）同源。这是通过构建在非洲爪蟾卵母细胞中有效转录的杂交5S RNA - tRNAPro和tRNAPro - 5S RNA基因来证明的。转录起始似乎是一个复杂的现象，其中内部启动子的两个组分都发挥作用。
