Darling J L, Oktar N, Thomas D G
Cell Biol Int Rep. 1983 Jan;7(1):23-30. doi: 10.1016/0309-1651(83)90101-7.
Multicellular tumour spheroids were prepared from a total of 46 human brain tumour biopsies by collagenase digestion and plating into agar coated flasks. Both primary malignant and secondary tumours formed spheroids with some correlation between the malignancy of tumour and the ability to undergo spheroid formation. The spheroids were capable of progressive growth, the rate of which was dependent, to some extent, on environmental conditions and was reflected by an increase in cell number within the spheroids. Spheroids prepared in this way may prove to be useful models for in vitro chemosensitivity and the general biology of brain tumours.
通过胶原酶消化并接种到涂有琼脂的培养瓶中，从总共46份人脑肿瘤活检样本中制备了多细胞肿瘤球体。原发性恶性肿瘤和继发性肿瘤均形成了球体，肿瘤的恶性程度与形成球体的能力之间存在一定相关性。这些球体能够进行渐进性生长，其生长速率在一定程度上取决于环境条件，并通过球体内细胞数量的增加得以体现。以这种方式制备的球体可能被证明是用于体外化学敏感性研究和脑肿瘤一般生物学研究的有用模型。
