Sale G E, Shulman H M, Hackman R C, Meyers J D
Diagn Gynecol Obstet. 1982 Winter;4(4):389-96.
We studied the standard frozen section as a diagnostic method for cytomegalovirus infection of the lung in bone marrow transplant recipients. Fifty-nine patients with cytomegalovirus pneumonia diagnosed by open lung biopsy were studied. The sensitivity of frozen section was compared with that of paraffin section, cytology of imprints prepared from the tissue sample, and viral culture. Fifteen percent of the cases were diagnosable at frozen section alone, and 44% by cytology alone. This confirms our previous study and indicates that frozen section unaided by more specific indentification methods provides low sensitivity even in cytomegalovirus-infected cells, which probably show the most easily identified cytopathic effects of any virus. This low sensitivity underscores the need for the additional sensitivity and specificity which may be contributed by immunolabeling techniques to rapid viral diagnosis.
我们研究了标准冰冻切片作为骨髓移植受者肺部巨细胞病毒感染诊断方法的可行性。研究对象为59例经开胸肺活检确诊为巨细胞病毒肺炎的患者。将冰冻切片的敏感性与石蜡切片、组织样本制备的印片细胞学检查及病毒培养的敏感性进行了比较。15%的病例仅通过冰冻切片即可诊断，44%的病例仅通过细胞学检查即可诊断。这证实了我们之前的研究，并表明即使在巨细胞病毒感染的细胞中，在没有更特异鉴定方法辅助的情况下，冰冻切片的敏感性也较低，而巨细胞病毒感染的细胞可能显示出任何病毒中最易识别的细胞病变效应。这种低敏感性凸显了免疫标记技术对快速病毒诊断可能提供的额外敏感性和特异性的必要性。
