Beaugé F, Fleuret-Balter C, Barin F, Nordmann J, Nordmann R
Drug Alcohol Depend. 1982 Oct-Nov;10(2-3):143-51. doi: 10.1016/0376-8716(82)90005-9.
Crude synaptic membrane fluidity (checked by fluorescence polarization) together with (Na+ + K+) ATPase activity were examined 18 hours after a single oral ethanol administration (5 g/kg bwt.) to naive rats and to rats previously intubated with ethanol repeatedly during 4 days. The sensibility of both parameters to different concentrations of ethanol added in vitro (0.175 M-1.400 M) was also determined. Although no changes in the basal intrinsic fluidity were found, (Na+ + K+)ATPase activity increased slightly in both conditions. The fluidizing as well as the ATPase inhibiting effects following the addition of ethanol in vitro were markedly increased 18 hours after ethanol administration to naive rats. This hypersensitization was no longer apparent in rats pretreated with ethanol during 4 days. The acute ethanol-induced hypersensitization found in naive rats appears not to be related to an unspecific stress or to changes in body temperature. The disappearance of this hypersensitization in short-term alcohol-intoxicated animals may represent the first stage of tolerance acquisition.
在单次口服乙醇（5克/千克体重）18小时后，对未接触过乙醇的大鼠以及此前4天内反复经插管给予乙醇的大鼠，检测其粗制突触膜流动性（通过荧光偏振检测）以及（钠+钾）ATP酶活性。还测定了这两个参数对体外添加不同浓度乙醇（0.175 M - 1.400 M）的敏感性。尽管未发现基础内在流动性有变化，但在两种情况下（钠+钾）ATP酶活性均略有增加。在未接触过乙醇的大鼠给予乙醇18小时后，体外添加乙醇后的流化作用以及ATP酶抑制作用均显著增强。在经4天乙醇预处理的大鼠中，这种超敏反应不再明显。在未接触过乙醇的大鼠中发现的急性乙醇诱导的超敏反应似乎与非特异性应激或体温变化无关。短期酒精中毒动物中这种超敏反应的消失可能代表了耐受性获得的第一阶段。
