Davies R J, Diamond S P
Biochim Biophys Acta. 1978 Mar 29;518(1):1-6. doi: 10.1016/0005-2787(78)90110-7.
Under conditions where it is single-stranded (pH 7.0, 0.05 M Na+), poly(inosinic acid) complexes guanosine and 2'-deoxyguanosine in a strongly cooperative process that ultimately results in gel formation. The binding isotherm for formation of the poly(I).deoxyguanosine complex at 3.5 degrees C shows that it contains approximately one molecule of deoxyguanosine bound per IMP residue of the poly(I). This is compatible with the complex having a structure similar to that of the four-stranded secondary structure of poly(I) in which two of the poly(I) strands are replaced by helical arrays of deoxyguanosine molecules. The complex is laevorotatory at wavelengths longer than 300 nm. Optical rotation and ultraviolet absorbance versus temperature profiles show that on thermal dissociation the complex undergoes a co-operative helix-coil transition with Tm in the range 10--20 degrees C.
在单链状态下（pH 7.0，0.05 M Na⁺），聚肌苷酸通过一个高度协同的过程与鸟苷和2'-脱氧鸟苷结合，最终导致凝胶形成。在3.5℃下形成聚（I）-脱氧鸟苷复合物的结合等温线表明，每一个聚（I）的IMP残基大约结合一个脱氧鸟苷分子。这与该复合物具有类似于聚（I）的四链二级结构的结构相一致，其中两条聚（I）链被脱氧鸟苷分子的螺旋阵列所取代。该复合物在波长大于300 nm时呈左旋。旋光性和紫外吸光度随温度变化的曲线表明，在热解离时，该复合物经历了一个协同的螺旋-线圈转变，其熔解温度（Tm）在10-20℃范围内。
