McTavish C F, Nelson W J, Traub P
FEBS Lett. 1983 Apr 18;154(2):251-6. doi: 10.1016/0014-5793(83)80159-8.
The turnover of vimentin and vimentin-derived peptides has been examined in logarithmically growing Ehrlich ascites tumour cells. Cells were pulse-labelled with [35S]methionine for 30 min and then chased for up to 60 h. It was found that the specific radioactivity of the main isoelectric variant of vimentin decreased to half the original value in 15.3 h which was close to the division time of the cells (16 h). The protein moiety of the phosphorylated variant of vimentin also turned over very slowly, in contrast to the turnover rate of the phosphate group itself which has a half-life of 1.4 h. The role of the intermediate filament-specific, Ca2+-activated proteinase has been considered in relationship to the slow turnover of vimentin.
在对数生长期的艾氏腹水瘤细胞中检测了波形蛋白及其衍生肽段的周转情况。细胞用[35S]甲硫氨酸脉冲标记30分钟，然后追踪长达60小时。结果发现，波形蛋白主要等电变体的比放射性在15.3小时内降至原始值的一半，这与细胞的分裂时间（16小时）相近。波形蛋白磷酸化变体的蛋白质部分周转也非常缓慢，与之形成对比的是，磷酸基团本身的周转速率半衰期为1.4小时。已经探讨了中间丝特异性的、Ca2+激活的蛋白酶在波形蛋白缓慢周转方面的作用。
