Cífková I, Sprincl L
J Biomed Mater Res. 1980 Nov;14(6):723-30. doi: 10.1002/jbm.820140604.
Pyrophosphate labeled with 99Tc was evaluated as an indicator of the calcification which occurs after implantation of polymeric materials in rats. Poly(2-hydroxyethyl methacrylate), both porous and homogeneous, had been implanted. 99Tc pyrophosphate was applied intravenously to rats in intervals from 14 days to 15 months. Scintigraphy, x-ray examination, and histology were carried out. The ratio of specific activity in the capsule around the implant to specific activity in the reference tissue was determined. The cumulation of activity was parallel with the development of calcification as revealed by histology. The method can be used not only in the screening test of new polymeric materials, but also in a quantitative determination of the degree of calcification in general.
用99Tc标记的焦磷酸盐被评估为大鼠植入聚合材料后发生钙化的指标。已植入多孔和均质的聚甲基丙烯酸2-羟乙酯。在14天至15个月的间隔内，将99Tc焦磷酸盐静脉注射给大鼠。进行了闪烁扫描、x光检查和组织学检查。测定了植入物周围包膜中的比活度与参考组织中的比活度之比。组织学显示，活性的累积与钙化的发展平行。该方法不仅可用于新型聚合材料的筛选试验，还可用于一般钙化程度的定量测定。
