Abel M H, Kelly R W
J Clin Endocrinol Metab. 1983 Apr;56(4):678-85. doi: 10.1210/jcem-56-4-678.
We investigated the ability of the nonpregnant human uterus to metabolize prostaglandins E2 and F2 alpha (PGE2 and PGF2 alpha) using radiolabeled substrates together with gas chromatography-mass spectrometry for the measurement of specific metabolites. Both the 15-hydroxyprostaglandin dehydrogenase and the delta 13-reductase enzymes are present in endometrium and myometrium. As shown for other tissues, the dehydrogenase is NAD+ dependent, while the delta 13-reductase requires NADPH as cofactor. PGE2 was the preferred substrate in both tissues; however, the metabolizing capacity of follicular phase myometrium was comparable with that of midluteal phase endometrium. The Km values of the dehydrogenase for PGE2 and PGF2 alpha were similar in both endometrium (1.89 X 10(-6) and 18.52 X 10(-6) mol/liter, respectively) and myometrium (2.76 X 10(-6) and 11.64 X 10(-6) mol/liter, respectively), suggesting that the same enzyme is present in both tissues although the regulation of this enzyme may differ in endometrium and myometrium.
我们使用放射性标记底物结合气相色谱 - 质谱法来测量特定代谢物，研究了未孕人类子宫代谢前列腺素E2和F2α（PGE2和PGF2α）的能力。15 - 羟基前列腺素脱氢酶和δ13 - 还原酶在子宫内膜和子宫肌层中均有存在。正如在其他组织中所显示的，脱氢酶依赖于NAD +，而δ13 - 还原酶需要NADPH作为辅助因子。PGE2是两种组织中的首选底物；然而，卵泡期子宫肌层的代谢能力与黄体中期子宫内膜的代谢能力相当。脱氢酶对PGE2和PGF2α的Km值在子宫内膜（分别为1.89×10（-6）和18.52×10（-6）mol /升）和子宫肌层（分别为2.76×10（-6）和11.64×10（-6）mol /升）中相似，这表明两种组织中存在相同的酶，尽管该酶在子宫内膜和子宫肌层中的调节可能不同。
