Chatterjee N K, Samsonoff W A, Tuchowski C
J Virol. 1983 Feb;45(2):832-41. doi: 10.1128/JVI.45.2.832-841.1983.
HeLa cells infected with several group B coxsackieviruses contain a previously undetected, virus-specific ribonucleoprotein particle which we designated membrane-bound virion (MBV). MBVs of B5 virus have a pronounced polygonal appearance and are slightly smaller than virions. The particles sediment more slowly (at about 107S) and have a lower buoyant density (about 1.30). They contain 35S virion RNA; only three, and not four, capsid proteins; and at least seven additional proteins with apparent molecular weights of 21,000 to 92,000. Three of the latter proteins appear to be of host origin; the rest may be precursors of virion capsid proteins. The RNA is resistant to digestion by RNase, and EDTA treatment disrupts the particle. MBVs are infectious, although significantly less so than virions. Cells infected with MBVs produce both types of progeny, virions and MBVs. In coinfected cultures, the yield of progeny is lower than in cells infected with virions alone, suggesting interference by MBVs. Synthesis of both types can be detected within 3.5 h after infection, and synthesis continues for 24 h.
感染了几种B组柯萨奇病毒的海拉细胞含有一种以前未被检测到的、病毒特异性核糖核蛋白颗粒，我们将其命名为膜结合病毒体（MBV）。B5病毒的MBV呈明显的多边形外观，比病毒体略小。这些颗粒沉降较慢（约107S），浮力密度较低（约1.30）。它们含有35S病毒体RNA；只有三种而非四种衣壳蛋白；以及至少七种表观分子量为21,000至92,000的其他蛋白质。后三种蛋白质似乎来源于宿主；其余的可能是病毒体衣壳蛋白的前体。RNA对核糖核酸酶的消化具有抗性，而乙二胺四乙酸（EDTA）处理会破坏该颗粒。MBV具有传染性，尽管其传染性明显低于病毒体。感染MBV的细胞会产生两种类型的子代，即病毒体和MBV。在共感染培养物中，子代的产量低于仅感染病毒体的细胞，这表明MBV存在干扰作用。两种类型的合成在感染后3.5小时内均可检测到，并且合成会持续24小时。
