Andreesen R, Munder P G
J Natl Cancer Inst. 1983 Apr;70(4):623-7.
Sera collected from 1 tumor patient after a 12-hour infusion of 30-50 mg alkyl lysophospholipids (ALP)/kg induced a progressive destruction of human leukemia cells (HL60) in vitro. This cytotoxic serum activity correlated with the dose of ALP administered and was inhibited by the addition of a metabolizable lysophospholipid analogue. Human bone marrow cells and concanavalin A-stimulated lymphoblasts were affected to a much lesser degree, whereas cells of the erythroleukemia line K562 appeared to be relatively resistant. When cells were cultured in postinfusion sera, the cytotoxicity of ALP in vitro was enhanced as much as fifteenfold when compared with the cytotoxicity of ALP when the cells were cultured in normal sera. No change in either relative or absolute distribution of phospholipids in postinfusion sera could be detected.
从1例肿瘤患者体内采集的血清，该患者在输注30 - 50毫克烷基溶血磷脂（ALP）/千克12小时后，其血清在体外可诱导人白血病细胞（HL60）逐渐被破坏。这种细胞毒性血清活性与所给予的ALP剂量相关，并可通过添加可代谢的溶血磷脂类似物而受到抑制。人骨髓细胞和伴刀豆球蛋白A刺激的淋巴母细胞受影响程度小得多，而红白血病细胞系K562的细胞似乎相对耐药。当细胞在输注后血清中培养时，与在正常血清中培养细胞时ALP的细胞毒性相比，ALP在体外的细胞毒性增强了多达15倍。在输注后血清中未检测到磷脂的相对或绝对分布变化。
