早期人胎盘中β-肾上腺素能受体的[3H]-二氢阿普洛尔结合特性研究

Beta-adrenergic receptors in early human placenta characterization of [3H]-dihydroalprenolol binding.

作者信息

Falkay G, Kovacs L

出版信息

Life Sci. 1983 Apr 4;32(14):1583-90. doi: 10.1016/0024-3205(83)90864-0.

Abstract

The binding characteristics of beta-adrenergic ligand [3H]-dihydroalprenolol (DHA) were determined in particulate membranes of early human placenta (8-12 weeks of gestation). [3H]-DHA binding to crude membrane fractions was rapid, reversible, saturable and linearly correlated with the membrane protein concentration. Scatchard analysis of saturation experiments showed a KD of 2.80 +/- 0.9 nM and a density of binding sites of 330.30 +/- 93.5 fmol/mg protein. Agonist potency isoproterenol greater than epinephrine greater than norepinephrine indicated that early human placenta contains an adrenergic receptor of beta-2 subtype.

摘要

在人早期胎盘（妊娠8 - 12周）的微粒体膜中测定了β-肾上腺素能配体[3H]-二氢烯丙洛尔（DHA）的结合特性。[3H]-DHA与粗膜组分的结合迅速、可逆、可饱和，且与膜蛋白浓度呈线性相关。饱和实验的Scatchard分析显示解离常数（KD）为2.80±0.9 nM，结合位点密度为330.30±93.5 fmol/mg蛋白。激动剂效力异丙肾上腺素＞肾上腺素＞去甲肾上腺素，表明人早期胎盘含有β-2亚型的肾上腺素能受体。