Fehlhaber H W, Metternich K, Tripier D, Uihlein M
Biomed Mass Spectrom. 1978 Mar;5(3):188-91. doi: 10.1002/bms.1200050305.
A method has been developed for the blood level determination of the antihypertensive agent tiamenidine hydrochloride. The serum samples are mixed with deuterium labelled tiamenidine hydrochloride as an internal standard and extracted with methylene chloride. The extracts are derivatized with heptafluorobutyric acid anhydride and analysed by means of gas chromatography mass spectrometry using the selected ion monitoring technique to measure the molecular ion intensities of the bis-heptafluorobutyryl derivatives of tiamenidine hydrochloride and of the internal standard. Using 5 ml serum, the limit of detection is 0.2 ng ml-1 with an accuracy of +/- 0.17 ng (Syx of the calibration curve).
已开发出一种用于测定抗高血压药物盐酸噻美尼定血药浓度的方法。血清样本与作为内标的氘代盐酸噻美尼定混合，并用二氯甲烷萃取。萃取物用七氟丁酸酐衍生化，然后采用选择离子监测技术通过气相色谱 - 质谱联用进行分析，以测量盐酸噻美尼定和内标的双七氟丁酰衍生物的分子离子强度。使用5ml血清时，检测限为0.2ng/ml，准确度为±0.17ng（校准曲线的Syx）。
