当DNA处于溶液中时碱基倾斜的流动二色性证据。
Flow dichroism evidence for tilting of the bases when DNA is in solution.
作者信息
Dougherty A M, Causley G C, Johnson W C
出版信息
Proc Natl Acad Sci U S A. 1983 Apr;80(8):2193-5. doi: 10.1073/pnas.80.8.2193.
Abstract
Flow linear dichroism studies of DNAs from Clostridium perfringens, Escherichia coli, and Micrococcus luteus are extended into the vacuum UV region to 175 nm. The short-wavelength pi pi region shows a less negative reduced linear dichroism than the well-studied long-wavelength pi pi region. These results are independent of the type of DNA and the concentration of salt over the range 0.001 to 1.0 M. This proves that the bases are not perpendicular to the flow orientation. Assuming that the DNA tends to align along its helix axis, the largest angle that the bases can make with the helix axis is 73 +/- 3 degrees.
摘要
对产气荚膜梭菌、大肠杆菌和藤黄微球菌的DNA进行的流动线性二色性研究扩展到了真空紫外区域，直至175纳米。与研究充分的长波长π-π区域相比，短波长π-π区域的还原线性二色性负值较小。这些结果与DNA的类型以及0.001至1.0 M范围内盐的浓度无关。这证明碱基并不垂直于流动方向。假设DNA倾向于沿其螺旋轴排列，碱基与螺旋轴能形成的最大角度为73±3度。
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