1型马疱疹病毒（EHV1）和1型马鼻病毒（ERhV1）经β-丙内酯（BPL）和紫外线（UV）灭活后的免疫原性。

Immunogenicity of equine herpesvirus type 1 (EHV1) and equine rhinovirus type 1 (ERhV1) following inactivation by betapropiolactone (BPL) and ultraviolet (UV) light.

作者信息

Campbell T M, Studdert M J, Blackney M H

出版信息

Vet Microbiol. 1982 Dec;7(6):535-44. doi: 10.1016/0378-1135(82)90047-5.

Abstract

Some kinetic data on the inactivation of equine herpesvirus type 1 (EHV1) and equine rhinovirus type 1 (ERhV1) by betapropiolactone (BPL) and ultraviolet (UV) irradiation are reported. 0.25% BPL at 37 degrees C for 1 h reduced the titre of EHV1 by greater than 10(3 . 4) and of ERhV1 by greater than 10(4 . 1) TCID50/ml. UV irradiation (334 microW/cm2) produced similar reductions in titre after 2 min. These data were used as a basis for inactivating EHV1 and ERhV1 by the combined action of BPL and UV irradiation. Viruses were exposed to 0.1% BPL for 1 h at 4 degrees C with constant stirring, followed by UV irradiation for 2 min, followed by incubation for 3 h at 37 degrees C. Inactivated EHV1 elicited secondary immune responses only in horses whereas ERhV1 produced primary immune responses in mice (including athymic nu/nu mice), rabbits and probably in horses.

摘要

报道了关于β-丙内酯（BPL）和紫外线（UV）照射对1型马疱疹病毒（EHV1）和1型马鼻病毒（ERhV1）灭活的一些动力学数据。37℃下0.25%的BPL作用1小时，使EHV1的滴度降低超过10(3.4)，使ERhV1的滴度降低超过10(4.1) TCID50/ml。紫外线照射（334微瓦/平方厘米）在2分钟后产生了类似的滴度降低。这些数据被用作通过BPL和紫外线照射的联合作用灭活EHV1和ERhV1的基础。病毒在4℃下以恒定搅拌暴露于0.1%的BPL中1小时，然后进行2分钟的紫外线照射，接着在37℃下孵育3小时。灭活的EHV1仅在马中引发二次免疫反应，而ERhV1在小鼠（包括无胸腺裸鼠）、兔子以及可能在马中产生初次免疫反应。