Beguinot F, Formisano S, Rotella C M, Kohn L D, Aloj S M
Biochem Biophys Res Commun. 1983 Jan 14;110(1):48-54. doi: 10.1016/0006-291x(83)91258-5.
Thyrotropin causes a rapid and significant increase in the fluorescence polarization of DPH when this hydrophobic probe is incorporated into a strain of functioning rat thyroid cells (FRTL5). This increase is ligand-specific and is not related to cAMP production. The phenomenon seems to reflect the interaction of thyrotropin with the glycoprotein component of its membrane receptor, as suggested by experiments in which thyrotropin causes increases in DPH fluorescence polarization in liposomes embedded with this receptor component but not with gangliosides. A strain of nonfunctioning rat thyroid cells (FRT), exhibiting no reactivity with monoclonal antibodies to the glycoprotein component of the thyrotropin receptor, requires two orders of magnitude higher concentrations of thyrotropin to exhibit a comparable phenomenon.
当将这种疏水性探针掺入有功能的大鼠甲状腺细胞系（FRTL5）时，促甲状腺激素会导致二棕榈酰磷脂酰胆碱（DPH）的荧光偏振迅速且显著增加。这种增加具有配体特异性，且与环磷酸腺苷（cAMP）的产生无关。该现象似乎反映了促甲状腺激素与其膜受体糖蛋白成分的相互作用，这一推测来自于以下实验结果：促甲状腺激素会使嵌入该受体成分而非神经节苷脂的脂质体中的DPH荧光偏振增加。一种无功能的大鼠甲状腺细胞系（FRT），对促甲状腺激素受体糖蛋白成分的单克隆抗体无反应，需要两个数量级更高浓度的促甲状腺激素才能表现出类似现象。
