Alkoutayni M, Stahl A J
Biochem Biophys Res Commun. 1983 Feb 10;110(3):945-50. doi: 10.1016/0006-291x(83)91054-9.
A yeast mitochondrial inner membrane hydrophobic protein 30K has been isolated and compared to subunit 32K of the yeast cytochrome bc 1 complex. Both proteins are translated on mitochondrial ribosomes, have nearly the same molecular weight and similar aminoacid compositions. Comparison was carried out by immunological techniques with specific antibodies, and by studying 3 yeast strains having mutations in the COB region of the mitochondrial DNA. Results show that the two proteins are not identical.
一种酵母线粒体内膜疏水蛋白30K已被分离出来,并与酵母细胞色素bc1复合物的32K亚基进行了比较。这两种蛋白质都在线粒体核糖体上翻译,分子量几乎相同,氨基酸组成也相似。通过使用特异性抗体的免疫技术以及研究线粒体DNA的COB区域发生突变的3种酵母菌株进行了比较。结果表明这两种蛋白质并不相同。