Tokimasa T, Cherubini E, North R A
Brain Res. 1983 Mar 14;263(1):57-62. doi: 10.1016/0006-8993(83)91200-3.
Intracellular recordings were made from S type neurons in the myenteric plexus of the guinea-pig ileum. Acetylcholine (ACh) was applied directly onto the soma membrane by iontophoresis with visual placement of the iontophoretic pipette. Fast nicotinic depolarizations were evoked which were followed by slow muscarinic depolarizations. After application of hyoscine to block the slow muscarinic depolarization, the nicotinic depolarization was found to be followed by a hyperpolarization. This hyperpolarization was several mV in amplitude and 1-5 s in total duration. It disappeared when the preceding nicotinic depolarization was blocked by hexamethonium. The ACh induced by hyperpolarization was due to calcium entry and subsequent opening of potassium channels.
在豚鼠回肠肌间神经丛的S型神经元上进行细胞内记录。通过离子电泳将乙酰胆碱（ACh）直接施加到胞体膜上，离子电泳移液管通过视觉定位放置。诱发快速烟碱样去极化，随后是缓慢的毒蕈碱样去极化。应用东莨菪碱阻断缓慢的毒蕈碱样去极化后，发现烟碱样去极化后紧接着是超极化。这种超极化幅度为几毫伏，总持续时间为1 - 5秒。当六甲铵阻断先前的烟碱样去极化时，它消失。超极化诱导的ACh是由于钙内流和随后钾通道的开放。
