Cavalieri E, Munhall A, Rogan E, Salmasi S, Patil K
Carcinogenesis. 1983;4(4):393-7. doi: 10.1093/carcin/4.4.393.
Benzo[a]pyrene (BP) and cyclopenteno[cd]pyrene (CPEP) are widespread environmental pollutants. CPEP is a relatively potent carcinogen in mouse skin with an activity second only to BP among environmental aromatic hydrocarbons. We have studied the combined application of BP and CPEP on mouse skin to determine their possible synergistic carcinogenic effect. Nine-week-old female Swiss mice in groups of 30 were treated on the back with high (H), medium (M) and low (L) doses, respectively, of 20 (H), 6.6 (M) or 2.2 (L) nmol BP or 200 (H), 66.6 (M) or 22.2 (L) nmol CPEP in 50 microliters acetone twice weekly for 48 weeks. Other groups received BP-H + CPEP-H, BP-M + CPEP-M, BP-L + CPEP-L, BP-H + CPEP-L, BP-M + CPEP-L, BP-L + CPEP-H, or BP-L + CPEP-M. A significant, 3- to 7-fold syncarcinogenic effect occurred when BP-M + CPEP-M were administered together. A smaller, but significant, synergistic effect (1.2- to 3.8-fold) was also observed when BP-M + CPEP-L or BP-L + CPEP-M was applied. Because of the syncarcinogenic effect of BP and CPEP, their abundance in engine emissions and ambient air samples may present a major source of carcinogenic risk.
苯并[a]芘（BP）和环戊烯并[cd]芘（CPEP）是广泛存在的环境污染物。CPEP在小鼠皮肤中是一种相对强效的致癌物，在环境芳烃中其活性仅次于BP。我们研究了BP和CPEP在小鼠皮肤上的联合应用，以确定它们可能的协同致癌作用。将30只一组的9周龄雌性瑞士小鼠背部分别用高（H）、中（M）和低（L）剂量的20（H）、6.6（M）或2.2（L）nmol BP或200（H）、66.6（M）或22.2（L）nmol CPEP溶于50微升丙酮中，每周两次，共处理48周。其他组接受BP-H + CPEP-H、BP-M + CPEP-M、BP-L + CPEP-L、BP-H + CPEP-L、BP-M + CPEP-L、BP-L + CPEP-H或BP-L + CPEP-M处理。当同时给予BP-M + CPEP-M时，出现了显著的3至7倍的协同致癌作用。当应用BP-M + CPEP-L或BP-L + CPEP-M时，也观察到了较小但显著的协同作用（1.2至3.8倍）。由于BP和CPEP的协同致癌作用，它们在发动机排放物和环境空气样本中的丰度可能是致癌风险的主要来源。
