Queen C
J Mol Appl Genet. 1983;2(1):1-10.
I have developed a plasmid vector pCQV2 for high-level expression of proteins in E. coli. The plasmid contains a very strong promoter and translation start point from bacteriophage lambda, and a restriction endonuclease site in which a gene may be placed under control of these initiation signals. The plasmid also contains the gene for a temperature-sensitive repressor of the lambda promoter, allowing 100-fold induction of protein expression. The vector pCQV2 has been used to synthesize SV40 small t antigen at the level of 5-10% of total E. coli protein, representing an order-of-magnitude improvement over previous methods.
我已经开发出一种质粒载体pCQV2，用于在大肠杆菌中高效表达蛋白质。该质粒包含一个来自噬菌体λ的非常强的启动子和翻译起始点，以及一个限制性内切酶位点，基因可以置于这些起始信号的控制之下。该质粒还包含λ启动子的温度敏感型阻遏物基因，可使蛋白质表达提高100倍。载体pCQV2已被用于合成SV40小t抗原，其水平达到大肠杆菌总蛋白的5%-10%，比以前的方法有了一个数量级的改进。
