Zolotukhin S B, Zhivolup A N, Krupskaia I V, Budmaska M I, Paton E B
Tsitol Genet. 1989 Nov-Dec;23(6):22-4.
A mutation in lac-operator region of pUC19 plasmid causing an increase in beta-galactosidase activity was observed. The plasmid was used as a vector to provide high level of expression of the cloned E. coli rplL gene.
观察到pUC19质粒的乳糖操纵子区域发生突变,导致β-半乳糖苷酶活性增加。该质粒用作载体,以提供克隆的大肠杆菌rplL基因的高水平表达。