Soft agar clonogenic assay in human breast cancer.

Relationship between histology, cloning efficiencies and estrogen receptors was studied in the group of breast cancer patient. Mechanical disaggregation gave poor cellular yields since only in 66% cells were ready for the bioassay. Comparison between clonogenic assay, histology and estrogen status of human breast cancer is of a limited value only, because of a small number of patients in our study.