Hwang-Lee L, Blamire J, Cottrell S F
Anal Biochem. 1983 Jan;128(1):47-53. doi: 10.1016/0003-2697(83)90342-1.
A method for the rapid isolation of mitochondrial DNA from the yeast Saccharomyces cerevisiae is described. Cells are first disrupted by vortexing with glass beads and the mitochondrial DNA is then extracted directly from the cell lysate by poly-L-lysine-kieselguhr-exchange chromatography. The method is unique from most other published procedures in that there is no requirement for the isolation of either a crude or purified mitochondrial preparation. Mitochondrial DNA isolated by this procedure is shown to yield restriction endonuclease fragment patterns identical to those obtained from DNA isolated by other previously reported procedures.
本文描述了一种从酿酒酵母中快速分离线粒体DNA的方法。首先通过与玻璃珠涡旋振荡来破碎细胞，然后通过聚-L-赖氨酸-硅藻土交换色谱法直接从细胞裂解物中提取线粒体DNA。该方法与大多数其他已发表的方法不同之处在于，无需分离粗制或纯化的线粒体提取物。通过该方法分离的线粒体DNA产生的限制性内切酶片段图谱与通过其他先前报道的方法分离的DNA所获得的图谱相同。
