Häggblad J, Eriksson H, Heilbronn E
J Neurochem. 1983 Jun;40(6):1581-4. doi: 10.1111/j.1471-4159.1983.tb08129.x.
A chemiluminescence method for the determination of endogenous acetylcholine was adapted to transmitter released from the hemidiaphragm of the rat. Released choline and acetylcholine were isolated and concentrated using KI3 precipitation. Between 1 and 20 pmol of acetylcholine may be measured without sample dilution. The detection limit for acetylcholine luminescence is 1.0 pmol; thus, with an observed precipitation recovery of approximately 30%, the lowest detectable released amount is 3.5 pmol. The limitations of the method are discussed.
一种用于测定内源性乙酰胆碱的化学发光方法被应用于大鼠半膈肌释放的递质。释放出的胆碱和乙酰胆碱通过KI3沉淀进行分离和浓缩。在不进行样品稀释的情况下，可以测定1至20皮摩尔的乙酰胆碱。乙酰胆碱发光的检测限为1.0皮摩尔；因此，观察到的沉淀回收率约为30%，最低可检测到的释放量为3.5皮摩尔。文中讨论了该方法的局限性。
