Szutowicz A, Morrison M R, Srere P A
J Neurochem. 1983 Jun;40(6):1664-70. doi: 10.1111/j.1471-4159.1983.tb08140.x.
Dibutyryl cyclic AMP and butyrate inhibited growth of S-20 (cholinergic) and NIE-115 (adrenergic) neuroblastoma clones. Both these drugs resulted in a parallel increase of choline acetyltransferase and ATP-citrate lyase activities in S-20 neuroblastoma cells. On the other hand, the increase in tyrosine hydroxylase activity in NIE-115 caused by these drugs was not accompanied by a significant change in ATP-citrate lyase activity. Both dibutyryl cyclic AMP and butyrate caused a decrease in fatty acid synthetase activity in both cell lines. The activities of pyruvate dehydrogenase, citrate synthase, choline acetyltransferase, and lactate dehydrogenase in both S-20 and NIE-115 cells were not significantly influenced by the drugs. ATP-citrate lyases from S-20 and NIE-115 had similar kinetic and immunological properties, and their subunits had the same molecular weight as the rat liver enzyme. These data indicate that the differential regulation of ATP-citrate lyase activity in cholinergic and adrenergic cells does not result from the existence of different molecular forms of the enzyme in these cell lines. They also provide further evidence to support the hypothesis that ATP-citrate lyase activity increases during maturation of normal cholinergic neurons and decreases in noncholinergic cells of the brain.
二丁酰环磷腺苷和丁酸盐抑制了S - 20（胆碱能）和NIE - 115（肾上腺素能）神经母细胞瘤克隆的生长。这两种药物都导致S - 20神经母细胞瘤细胞中胆碱乙酰转移酶和ATP - 柠檬酸裂解酶活性平行增加。另一方面，这些药物引起的NIE - 115中酪氨酸羟化酶活性增加并未伴随ATP - 柠檬酸裂解酶活性的显著变化。二丁酰环磷腺苷和丁酸盐均导致两种细胞系中脂肪酸合成酶活性降低。S - 20和NIE - 115细胞中的丙酮酸脱氢酶、柠檬酸合酶、胆碱乙酰转移酶和乳酸脱氢酶活性均未受到药物的显著影响。来自S - 20和NIE - 115的ATP - 柠檬酸裂解酶具有相似的动力学和免疫学特性，其亚基的分子量与大鼠肝脏酶相同。这些数据表明，胆碱能和肾上腺素能细胞中ATP - 柠檬酸裂解酶活性的差异调节并非源于这些细胞系中该酶存在不同的分子形式。它们还提供了进一步证据支持以下假说：正常胆碱能神经元成熟过程中ATP - 柠檬酸裂解酶活性增加，而脑内非胆碱能细胞中该酶活性降低。
