Kochetkov S N, Bagirov E M, Gabibov A G, Severin E S
Mol Biol (Mosk). 1983 Mar-Apr;17(2):339-44.
The reaction of the solvolysis of the phosphointermediate, formed in the course of the phosphotransferase reaction was studied using a number of non-specific nucleophilic agents. The methods of identification of the solvolysis products and determination of kinetic parameters were developed. The estimated dependence of the reaction of the reactivity of the nucleophilic agents versus their basicity allowed some conclusions about the transition state structure in the cases of enzyme's interaction with nucleophilic agents and protein substrate. The energy contribution of the specific interaction of the enzyme with histone H1 was evaluated.
使用多种非特异性亲核试剂研究了在磷酸转移酶反应过程中形成的磷酸中间体的溶剂解反应。开发了鉴定溶剂解产物和测定动力学参数的方法。通过估计亲核试剂反应性与其碱性之间的关系，得出了一些关于酶与亲核试剂及蛋白质底物相互作用时过渡态结构的结论。评估了酶与组蛋白H1特异性相互作用的能量贡献。
