Elli R, Marcucci L, Bosi R, Oppi C, Battaglia P A, Gigliani F
Nucleic Acids Res. 1983 Jun 11;11(11):3679-86. doi: 10.1093/nar/11.11.3679.
It has been recently reported that the pR plasmid enhances the UV survival in E.coli c600. In order to test whether this function may be expressed also in mammalian cells, LTA (tk- aprt-) mouse cells were cotransformed with pR plasmid DNA and ptk1 plasmid as selectable marker. Tk+ transformants were analyzed for their UV survival and for the presence of pR DNA sequences by blot-hybridization. The results show a correlation between the enhanced UV survival and presence of pR DNA sequences in cotransformed LTA mouse cells.
最近有报道称，pR质粒可提高大肠杆菌C600的紫外线存活率。为了测试该功能是否也能在哺乳动物细胞中表达，将LTA（tk- aprt-）小鼠细胞与pR质粒DNA和作为选择标记的ptk1质粒共转化。对tk+转化体进行紫外线存活率分析，并通过印迹杂交检测pR DNA序列的存在情况。结果表明，在共转化的LTA小鼠细胞中，紫外线存活率的提高与pR DNA序列的存在之间存在相关性。
