采用酶联免疫吸附测定法检测尿液中的人乳头多瘤空泡病毒BKV和JCV。
Detection of human papovaviruses BKV and JCV in urines by ELISA.
作者信息
Arthur R R, Shah K V, Yolken R H, Charache P
出版信息
Prog Clin Biol Res. 1983;105:169-76.
PMID:6304754
Abstract
A double-antibody indirect ELISA using a chromogenic substrate and a single-antibody sandwich direct ELISA using chromogenic and fluorogenic substrates were developed for the detection of the human papovaviruses BKV and JCV. All three assays were comparable in their sensitivity. BKV was detected in urine from 12 of 63 (19%) highly immunocompromised patients and JCV in urine from 4 of 31 (12.9%) patients by the indirect method.
摘要
开发了一种使用显色底物的双抗体间接酶联免疫吸附测定法（ELISA）和一种使用显色及荧光底物的单抗体夹心直接ELISA，用于检测人乳头多瘤病毒BKV和JCV。所有这三种测定法在灵敏度方面具有可比性。通过间接法在63名（19%）高度免疫受损患者中的12人的尿液中检测到了BKV，在31名患者（12.9%）中的4人的尿液中检测到了JCV。
Zotero 插件