Hamilton R S, Gravell M, Major E O
Laboratory of Molecular Medicine, National Institute of Neurological Disorders and Stroke, Bethesda, Maryland 20892-4164, USA.
J Clin Microbiol. 2000 Jan;38(1):105-9. doi: 10.1128/JCM.38.1.105-109.2000.
A comparison of antibody titers to JC virus (JCV) or BK virus (BKV) was made by hemagglutination inhibition (HI) and enzyme immunoassay (EIA) with 114 human plasma samples. Antibody titers to JCV or BKV determined by HI were lower than those determined by EIA. Nevertheless, as HI titers increased so did EIA titers. When antibody data were compared by the Spearman rank correlation test, highly significant correlations were found between HI and EIA titers. Results obtained by plotting EIA antibody titers for JCV against those for BKV generally showed a reciprocal relationship, i.e., samples with high antibody titers to JCV had lower antibody titers to BKV and vice versa. Some samples, however, had antibody titers to both viruses. Of the samples tested, 25.4% (25 of 114) had HI and EIA antibody titers to JCV and BKV which were identical or closely related. This is not the scenario one would expect for cross-reactive epitopes shared by the two viruses, but one suggesting that these samples were from individuals who had experienced infections by both viruses. Adsorption with concentrated JCV or BKV antigen of sera with high antibody titers to both JCV and BKV and testing by JCV and BKV EIA gave results which support this conclusion. Although 52.6% (51 of 97) of the samples from the Japanese population tested had very high antibody titers (>/=40,960) to either JCV or BKV, none of the samples were found by a dot blot immunoassay to have antibodies which cross-reacted with simian virus 40. The results from this study, in agreement with those of others, suggest that humans infected by JCV or BKV produce antibodies to species-specific epitopes on their VP1 capsid protein, which is associated with hemagglutination and cellular binding.
采用血凝抑制试验（HI）和酶免疫测定法（EIA）对114份人血浆样本进行了针对JC病毒（JCV）或BK病毒（BKV）的抗体滴度比较。通过HI测定的针对JCV或BKV的抗体滴度低于通过EIA测定的抗体滴度。然而，随着HI滴度升高，EIA滴度也升高。当通过Spearman秩相关检验比较抗体数据时，发现HI和EIA滴度之间存在高度显著的相关性。绘制JCV的EIA抗体滴度与BKV的EIA抗体滴度的结果通常显示出一种相互关系，即对JCV抗体滴度高的样本对BKV的抗体滴度较低，反之亦然。然而，一些样本对两种病毒都有抗体滴度。在所检测的样本中，25.4%（114份中的25份）针对JCV和BKV的HI和EIA抗体滴度相同或密切相关。这并非人们预期的两种病毒共享交叉反应表位的情况，而是表明这些样本来自曾感染过两种病毒的个体。用高抗体滴度的针对JCV和BKV的血清与浓缩的JCV或BKV抗原进行吸附，并通过JCV和BKV EIA检测，结果支持这一结论。尽管检测的日本人群样本中有52.6%（97份中的51份）对JCV或BKV具有非常高的抗体滴度（≥40960），但通过斑点印迹免疫测定法未发现任何样本具有与猴病毒40交叉反应的抗体。本研究结果与其他研究结果一致，表明感染JCV或BKV的人会产生针对其VP1衣壳蛋白上物种特异性表位的抗体，该蛋白与血凝和细胞结合有关。
