Imbert J, Lawrence J J, Birg F
Virology. 1983 Apr 30;126(2):711-6. doi: 10.1016/s0042-6822(83)80028-2.
The relationship between the cell cycle and the presence of the nuclear T antigen in simian virus 40-transformed rat cells independently derived from FR 3T3 cells has been investigated using a fluorescence-activated cell sorter and a combination of stainings for cellular DNA (4',6-diamidine-2-phenylindole dihydrochloride, DAPI) and for the viral T antigen (fluorescein-labeled IgG). Such an analysis revealed that in three out of six lines, T antigen could be detected only in the G2 phase of the cell cycle. In the other three lines, the accumulation of T antigen appeared to be independent of the position of the cell in the cycle. The emergence of either phenotype was strictly correlated with the physiological state of the cell (growing versus resting, respectively) at the time the transformants were established.
利用荧光激活细胞分选仪以及针对细胞DNA（4',6-二脒基-2-苯基吲哚二盐酸盐，DAPI）和病毒T抗原（荧光素标记的IgG）的联合染色，研究了从FR 3T3细胞独立衍生而来的猿猴病毒40转化大鼠细胞中细胞周期与核T抗原存在之间的关系。这样的分析表明，在六个细胞系中的三个中，仅在细胞周期的G2期能检测到T抗原。在另外三个细胞系中，T抗原的积累似乎与细胞在周期中的位置无关。这两种表型的出现都与建立转化体时细胞的生理状态（分别为生长状态与静止状态）密切相关。
