鼠伤寒沙门氏菌中烟酰胺腺嘌呤二核苷酸生物合成的一个不可或缺的基因。
An indispensable gene for NAD biosynthesis in Salmonella typhimurium.
作者信息
Hughes K T, Ladika D, Roth J R, Olivera B M
出版信息
J Bacteriol. 1983 Jul;155(1):213-21. doi: 10.1128/jb.155.1.213-221.1983.
Abstract
We have located the nadD locus between lip and leuS at 14 min on the Salmonella typhimurium chromosome, and we have shown it to be the structural gene for nicotinic acid mononucleotide adenylyltransferase. This is the first indispensable gene of pyridine nucleotide metabolism that has been identified. Mutants altered at this locus, isolated by their 6-aminonicotinamide resistance phenotype, accumulate abnormally large pools of nicotinic acid mononucleotide in vivo; many exhibit a temperature-sensitive lethal phenotype. Enzyme assays reveal markedly lower transferase activity in mutant extracts than in nadD+ extracts. The partial dominance of nadD mutants when placed in a nadD+/nadD diploid suggests that nicotinic acid mononucleotide adenylyltransferase is a multimeric enzyme.
摘要
我们已将鼠伤寒沙门氏菌染色体上位于lip和leuS之间、14分钟处的nadD基因座定位出来，并证明它是烟酰胺单核苷酸腺苷酸转移酶的结构基因。这是已鉴定出的吡啶核苷酸代谢中第一个不可或缺的基因。通过其对6-氨基烟酰胺的抗性表型分离出的该基因座发生改变的突变体，在体内积累了异常大量的烟酰胺单核苷酸池；许多突变体表现出温度敏感致死表型。酶活性测定显示，突变体提取物中的转移酶活性明显低于nadD+提取物中的活性。将nadD突变体置于nadD+/nadD二倍体中时其部分显性表明烟酰胺单核苷酸腺苷酸转移酶是一种多聚体酶。
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本文引用的文献
1
Hfr formation directed by tn10.
Genetics. 1979 Apr;91(4):639-55. doi: 10.1093/genetics/91.4.639.
2
Acetylornithinase of Escherichia coli: partial purification and some properties.
J Biol Chem. 1956 Jan;218(1):97-106.
3
Abstracts of papers presented at the 1980 meetings of the Genetic Society of America. Boulder, Colorado August 18-20, 1980.
Genetics. 1980;94(4 Pt 2 Suppl):1-16.
4
Nicotinamide adenine dinucleotide biosynthesis and pyridine nucleotide cycle metabolism in microbial systems.
Microbiol Rev. 1980 Mar;44(1):83-105. doi: 10.1128/mr.44.1.83-105.1980.
5
Linkage map of Escherichia coli K-12, edition 6.
Microbiol Rev. 1980 Mar;44(1):1-56. doi: 10.1128/mr.44.1.1-56.1980.
6
6-Aminonicotinamide-resistant mutants of Salmonella typhimurium.
J Bacteriol. 1983 Jun;154(3):1126-36. doi: 10.1128/jb.154.3.1126-1136.1983.
7
A method for detection of phage mutants with altered transducing ability.
Mol Gen Genet. 1971;110(4):378-81. doi: 10.1007/BF00438281.
8
Regulation of branched-chain amino acid biosynthesis in Salmonella typhimurium: isolation of regulatory mutants.
J Bacteriol. 1969 Mar;97(3):1272-82. doi: 10.1128/jb.97.3.1272-1282.1969.
9
Frameshift mutations.
Annu Rev Genet. 1974;8:319-46. doi: 10.1146/annurev.ge.08.120174.001535.
10
Specialized transduction of tetracycline resistance by phage P22 in Salmonella typhimurium. II. Properties of a high-frequency-transducing lysate.
Virology. 1972 Dec;50(3):883-98. doi: 10.1016/0042-6822(72)90442-4.
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