脑心肌炎病毒的缺陷干扰颗粒

Defective interfering particles of encephalomyocarditis virus.

作者信息

Radloff R J, Young S A

出版信息

J Gen Virol. 1983 Jul;64 (Pt 7):1637-41. doi: 10.1099/0022-1317-64-7-1637.

Abstract

Encephalomyocarditis (EMC) virus and five temperature-sensitive mutants of EMC virus were serially passed numerous times in HeLa, L929 and BHK-21 cells. Equilibrium centrifugation in CsCl density gradients of virus and electrophoresis of virus RNA from many of the high number passages indicated the presence of standard EMC virus and particles containing RNA of lower molecular weight. Three virus isolates with high passage number were able to interfere with the replication of the standard virions in mixed infections. We believe our results show the generation of defective interfering particles of EMC virus.

摘要

脑心肌炎（EMC）病毒及EMC病毒的五个温度敏感突变株在HeLa、L929和BHK - 21细胞中多次连续传代。对许多高传代数的病毒进行CsCl密度梯度平衡离心及病毒RNA电泳，结果表明存在标准EMC病毒及含有较低分子量RNA的颗粒。三个高传代数的病毒分离株在混合感染中能够干扰标准病毒粒子的复制。我们认为我们的结果显示了EMC病毒缺陷干扰颗粒的产生。