欧洲亚硝化单胞菌中的一氧化碳结合型c型细胞色素和一种高电位细胞色素c

CO-binding c-type cytochromes and a high-potential cytochrome c in Nitrosomonas europaea.

作者信息

Miller D J, Wood P M

出版信息

Biochem J. 1983 May 1;211(2):503-6. doi: 10.1042/bj2110503.

DOI:10.1042/bj2110503

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1154385/

Abstract

The purification of two soluble CO-binding cytochromes c from Nitrosomonas europaea is described. Cytochrome cCO-550 ran on sodium dodecyl sulphate/polyacrylamide-gel electrophoresis with an apparent Mr of 32 000, whereas for cytochrome cCO-552 the apparent Mr was 16 000. Redox potentials (Em, 7) were determined as +140 and -50mV respectively. Cytochrome cCO-550 was co-purified with a cytochrome c-553, for which an unusually high redox potential of +450mV was measured. These latter components were not resolved by gel-filtration chromatography or electrophoresis under denaturing conditions.

摘要

本文描述了从欧洲亚硝化单胞菌中纯化两种可溶性一氧化碳结合细胞色素c的方法。细胞色素cCO-550在十二烷基硫酸钠/聚丙烯酰胺凝胶电泳中的表观分子量为32000，而细胞色素cCO-552的表观分子量为16000。氧化还原电位（Em，7）分别测定为+140和-50mV。细胞色素cCO-550与细胞色素c-553共同纯化，测得其氧化还原电位异常高，为+450mV。在变性条件下，通过凝胶过滤色谱法或电泳无法分离这些后期组分。

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本文引用的文献

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Characterization of the c-type cytochromes of Nitrosomonas europaea with the aid of fluorescent gels.借助荧光凝胶对欧洲亚硝化单胞菌的c型细胞色素进行表征。

Biochem J. 1982 Dec 1;207(3):511-7. doi: 10.1042/bj2070511.

2

A P-450-like cytochrome and a soluble terminal oxidase identified as cytochrome o from Nitrosomonas europaea.一种类似细胞色素P-450的细胞色素和一种被鉴定为来自欧洲亚硝化单胞菌的细胞色素o的可溶性末端氧化酶。

Biochem Biophys Res Commun. 1965 Nov 8;21(3):248-56. doi: 10.1016/0006-291x(65)90279-2.

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Purification and properties of peroxidase from Nitrosomonas europaea.欧洲亚硝化单胞菌过氧化物酶的纯化及性质

J Bacteriol. 1968 Jul;96(1):93-7. doi: 10.1128/jb.96.1.93-97.1968.

4

Cytochrome c-552 and cytochrome c-554 derived from Nitrosomonas europaea. Purification, properties, and their function in hydroxylamine oxidation.源自欧洲亚硝化单胞菌的细胞色素c-552和细胞色素c-554。纯化、性质及其在羟胺氧化中的作用。

J Biochem. 1974 Jun;75(6):1265-73. doi: 10.1093/oxfordjournals.jbchem.a130510.

5

Purification of c-type cytochromes from Nitrosomonas europaea.

Biochim Biophys Acta. 1973 Jun 15;310(2):331-43. doi: 10.1016/0005-2795(73)90113-x.

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