Miller D J, Wood P M
Biochem J. 1983 May 1;211(2):503-6. doi: 10.1042/bj2110503.
The purification of two soluble CO-binding cytochromes c from Nitrosomonas europaea is described. Cytochrome cCO-550 ran on sodium dodecyl sulphate/polyacrylamide-gel electrophoresis with an apparent Mr of 32 000, whereas for cytochrome cCO-552 the apparent Mr was 16 000. Redox potentials (Em, 7) were determined as +140 and -50mV respectively. Cytochrome cCO-550 was co-purified with a cytochrome c-553, for which an unusually high redox potential of +450mV was measured. These latter components were not resolved by gel-filtration chromatography or electrophoresis under denaturing conditions.
本文描述了从欧洲亚硝化单胞菌中纯化两种可溶性一氧化碳结合细胞色素c的方法。细胞色素cCO-550在十二烷基硫酸钠/聚丙烯酰胺凝胶电泳中的表观分子量为32000，而细胞色素cCO-552的表观分子量为16000。氧化还原电位（Em，7）分别测定为+140和-50mV。细胞色素cCO-550与细胞色素c-553共同纯化，测得其氧化还原电位异常高，为+450mV。在变性条件下，通过凝胶过滤色谱法或电泳无法分离这些后期组分。
