Marion D, Wüthrich K
Biochem Biophys Res Commun. 1983 Jun 29;113(3):967-74. doi: 10.1016/0006-291x(83)91093-8.
Two-dimensional correlated spectroscopy (COSY) is used for measurements of proton-proton spin-spin coupling constants in protein 1H NMR spectra. High digital resolution along the frequency axis omega 2 is achieved by placing the carrier frequency in the center of the spectrum, using quadrature detection in both dimensions and presenting the spectrum in the phase sensitive mode. Compared to other techniques for studies of spin-spin coupling constants, COSY provides greatly improved spectral resolution. This is illustrated by experiments with H2O solutions of the small globular protein BUSI IIA (bull seminal inhibitor IIA).
二维相关光谱（COSY）用于测量蛋白质1H NMR谱中的质子-质子自旋-自旋耦合常数。通过将载频置于谱的中心、在两个维度上使用正交检测并以相敏模式呈现谱，可在频率轴ω2上实现高数字分辨率。与其他研究自旋-自旋耦合常数的技术相比，COSY提供了大大提高的光谱分辨率。小球蛋白BUSI IIA（牛精液抑制剂IIA）的H2O溶液实验说明了这一点。
