Bono A, Cantoro G, Martorana A, Palermo R, Pandolfo L
Biochim Biophys Acta. 1983 Jul 29;758(2):158-67. doi: 10.1016/0304-4165(83)90297-0.
Receptors for 125I-labelled human prolactin have been identified in the crude membrane fraction isolated from human ovarian tissue. The non-ionic detergent Triton X-100, has been used to solubilize the membrane fraction. The presence of the receptor in the detergent extract was demonstrated by gel filtration and sucrose density gradient centrifugation. The binding was time-temperature dependent, being maximal at 23 degrees C after 15 h of incubation. Large amounts of other peptide hormones did not inhibit the binding of 125I-labelled human prolactin. The binding Scatchard analysis demonstrated that the affinity of the soluble receptor (Ka 1.13 +/- 0.15 X 10(10) M-1) for the labelled hormone was slightly greater than that of the crude membrane fraction (Ka 0.91 +/- 0.12 X 10(10) M-1). The binding capacity of the solubilized receptor was also significantly greater than that seen in the particulate before solubilization. The apparent Stokes radius of the solubilized receptor was estimated to be 57 A and that the hormone-receptor complex 60 A. The sedimentation coefficient of the solubilized receptor was 7.0 +/- 0.1 s, whereas that of the hormone-receptor complex was 7.5 +/- 0.2 s.
在从人卵巢组织分离出的粗膜部分中已鉴定出125I标记的人催乳素受体。非离子去污剂Triton X - 100已被用于溶解膜部分。通过凝胶过滤和蔗糖密度梯度离心证明了去污剂提取物中存在受体。结合具有时间 - 温度依赖性，在23℃孵育15小时后达到最大值。大量其他肽激素不抑制125I标记的人催乳素的结合。结合的Scatchard分析表明，可溶性受体（Ka 1.13±0.15×10(10) M-1）对标记激素的亲和力略大于粗膜部分（Ka 0.91±0.12×10(10) M-1）。可溶性受体的结合能力也明显大于溶解前颗粒中的结合能力。估计可溶性受体的表观斯托克斯半径为57 Å，激素 - 受体复合物为60 Å。可溶性受体的沉降系数为7.0±0.1 s，而激素 - 受体复合物的沉降系数为7.5±0.2 s。
