Donald R A, Redekopp C, Cameron V, Nicholls M G, Bolton J, Livesey J, Espiner E A, Rivier J, Vale W
Endocrinology. 1983 Sep;113(3):866-70. doi: 10.1210/endo-113-3-866.
The 41-residue ovine corticotropin releasing factor (CRF) was administered iv and intracerebroventricularly (icv) to merino sheep. A significant rise in plasma ACTH, beta-lipotropin (beta LPH) and cortisol was demonstrated after the administration of 200 micrograms, iv. A highly significant correlation between the increments in plasma ACTH and beta LPH was observed. The plasma ACTH rise was evident within 5 min and was abolished by the prior administration of 0.4-4.0 mg dexamethasone. No significant rise in plasma GH, LH, PRL, insulin, glucagon, pancreatic polypeptide, met-enkephalin, angiotensin II, aldosterone, or vasopressin could be demonstrated. Although smaller doses of CRF (50 ng to 5 micrograms) were effective when given icv, the ACTH response was more delayed. It is concluded that CRF stimulates a rapid increase in the secretion of ACTH and beta LPH in sheep. Suppression of this response by dexamethasone indicates that glucocorticoids are capable of acting on the pituitary to inhibit the ACTH response to CRF. The delayed response when CRF is given icv may be due to diffusion. The action of CRF appears to be relatively specific, in that the plasma concentrations of the other pancreatic, pituitary, and adrenal hormones measured were not affected.
将41个氨基酸残基的绵羊促肾上腺皮质激素释放因子（CRF）经静脉内和脑室内（icv）给予美利奴绵羊。静脉注射200微克后，血浆促肾上腺皮质激素（ACTH）、β-促脂素（βLPH）和皮质醇显著升高。观察到血浆ACTH和βLPH的增量之间存在高度显著的相关性。血浆ACTH在5分钟内升高，并被预先给予0.4 - 4.0毫克地塞米松所消除。未发现血浆生长激素（GH）、促黄体生成素（LH）、催乳素（PRL）、胰岛素、胰高血糖素、胰多肽、甲硫氨酸脑啡肽、血管紧张素II、醛固酮或血管加压素显著升高。虽然较小剂量的CRF（50纳克至5微克）经脑室内给药有效，但ACTH反应更延迟。结论是CRF刺激绵羊ACTH和βLPH分泌迅速增加。地塞米松对这种反应的抑制表明糖皮质激素能够作用于垂体以抑制ACTH对CRF的反应。CRF经脑室内给药时反应延迟可能是由于扩散。CRF的作用似乎相对特异，因为所测定的其他胰腺、垂体和肾上腺激素的血浆浓度未受影响。
