MacDonald M J, Warner T F, Pellett J R
J Clin Endocrinol Metab. 1983 Sep;57(3):662-4. doi: 10.1210/jcem-57-3-662.
The activities of flavin-linked, mitochondrial glycerol phosphate dehydrogenase in islet cell adenomas from two infants were 8 and 16 times higher than those in their uninvolved pancreases. The enzyme in the insulinomas, but not that in the nearby pancreases, was inhibited by diazoxide, which is a known inhibitor of insulin secretion in vitro and in vivo. These results provide preliminary evidence that the enzyme may be used as an adjunct to histology for identifying human insulin-secreting tissues. Its sensitivity to diazoxide adds specificity to the test. Taken together with previous work with animal pancreatic islets, the results of the present study suggest that the glycerol phosphate shuttle plays an important role in stimulus-secretion coupling in the pancreatic beta-cell.
两名婴儿胰岛细胞腺瘤中黄素连接的线粒体甘油磷酸脱氢酶活性分别比其未受累胰腺中的活性高8倍和16倍。二氮嗪可抑制胰岛素瘤中的该酶，但不抑制附近胰腺中的该酶，二氮嗪在体外和体内均是已知的胰岛素分泌抑制剂。这些结果提供了初步证据，表明该酶可用作组织学的辅助手段来识别人类胰岛素分泌组织。其对二氮嗪的敏感性增加了检测的特异性。结合先前对动物胰岛的研究工作，本研究结果表明甘油磷酸穿梭在胰腺β细胞的刺激-分泌偶联中起重要作用。
