[DNA与限制性内切酶SalGI复合物的电子显微镜研究]

[Electron microscopy studies of DNA complexes with restriction endonuclease SalGI].

作者信息

Johannssen W

出版信息

Z Allg Mikrobiol. 1983;23(3):197-201. doi: 10.1002/jobm.3630230308.

Abstract

The binding properties of the type II restriction endonuclease SalGI to the plasmid DNA pGW 10 has been investigated by electron microscopic studies. Samples were spread by the BAC technique. In the presence of magnesium, SalGI binds as dimers and tetramers to the specific recognition site 5'-G-T-C-G-A-C-3' and with lower rate to the sequence 5'-G-T-C-A-A-C-3', which represents the recognition site of the restriction endonucleases Hind II and Hinc II.

摘要

通过电子显微镜研究了II型限制性内切酶SalGI与质粒DNA pGW 10的结合特性。样品采用BAC技术铺展。在镁存在的情况下，SalGI以二聚体和四聚体形式结合到特定识别位点5'-G-T-C-G-A-C-3'，并以较低速率结合到序列5'-G-T-C-A-A-C-3'，该序列代表限制性内切酶Hind II和Hinc II的识别位点。