Zaprianova E, Dikov A, Christova M, Kadiisky D
Acta Histochem. 1983;72(2):161-6. doi: 10.1016/S0065-1281(83)80049-X.
A histochemical method for demonstration of 2',3'-cyclic nucleotide 3'-phosphohydrolase activity is proposed. Cryostat sections, fixed in chlorophorm-methanol mixture, are incubated in a solution containing cyclic adenosine 2',3'-monophosphate, acid phosphatase, lead nitrate in acetate buffer, pH = 6,4 or 6.5. This method is based on conversion by the 2',3'-cyclic nucleotide 3'-phosphohydrolase of cyclic adenosine 2',3'-monophosphate to nucleotide-2'-monophosphate. Under the action of exogenous acid phosphatase, inorganic phosphate is released. It precipitates as lead phosphate if lead ions are present. By adding yellow ammonium sulphide, the lead phosphate is converted to lead sulphide which is the visible reaction product.
提出了一种用于显示2',3'-环核苷酸3'-磷酸水解酶活性的组织化学方法。将冷冻切片固定在氯仿 - 甲醇混合物中，然后在含有2',3'-环磷酸腺苷、酸性磷酸酶、乙酸缓冲液（pH = 6.4或6.5）中的硝酸铅的溶液中孵育。该方法基于2',3'-环核苷酸3'-磷酸水解酶将2',3'-环磷酸腺苷转化为核苷酸-2'-单磷酸。在外源酸性磷酸酶的作用下，释放出无机磷酸盐。如果存在铅离子，它会沉淀为磷酸铅。通过加入黄色硫化铵，磷酸铅转化为硫化铅，这就是可见的反应产物。
