Raynal F, Michot B, Bachellerie J P
FEBS Lett. 1983 Sep 5;161(1):135-9. doi: 10.1016/0014-5793(83)80746-7.
Among the stepwise cleavage reactions involved in the processing of rRNA precursors in mammalian cells, an early event corresponds to the removal of the so-called 'external transcribed regions' which are located upstream 18 S rRNA sequence within the primary transcript. We have determined the primary sequence of the domain of mouse pre-rRNA which encompasses this early processing site and analyzed its structural features with reference to the other eukaryotic homologs available. The potential involvement of secondary structure features of rRNA precursors in the recognition process for cleavage is discussed.
在哺乳动物细胞中rRNA前体加工所涉及的逐步切割反应中,早期事件是去除所谓的“外部转录区域”,这些区域位于初级转录本中18 S rRNA序列的上游。我们已经确定了小鼠前体rRNA中包含这个早期加工位点的结构域的一级序列,并参照现有的其他真核生物同源序列分析了其结构特征。文中还讨论了rRNA前体二级结构特征在切割识别过程中的潜在作用。