Von Behren L A, Chaudhary S, Khardori N, Rabinovich S, Shu M D, Tewari R P
J Reticuloendothel Soc. 1983 Aug;34(2):99-111.
The role of macrophages in the innate immunity of mice to histoplasmosis was investigated using silica, which selectively inactivates macrophages. Mice given silica IV 1 day prior to challenge with live yeast cells of Histoplasma capsulatum were more susceptible to infection than were untreated controls. This increased susceptibility to Histoplasma was observed when mice were given silica at 1, 14, and 21 days prior to infection but not at 3 and 7 days. Silica treated mice that survived 30 days after challenge with a sublethal dose of Histoplasma had 23 times more viable organisms in their spleens than in those of untreated controls. The blastogenic response of spleen cells to concanavalin A and phytohemagglutinin was unaffected at 12 hr after silica injection but was significantly depressed between 1 and 21 days. In contrast, silica treatment did not affect the blastogenic response of spleen cells to lipopolysaccharide. Silica particles were cytotoxic for mouse peritoneal macrophages but not to lymphocytes in vitro. These results indicate that macrophages play an essential role in natural immunity to histoplasmosis.
利用可选择性使巨噬细胞失活的二氧化硅，研究了巨噬细胞在小鼠对组织胞浆菌病天然免疫中的作用。在接种荚膜组织胞浆菌活酵母细胞前1天经静脉给予二氧化硅的小鼠，比未处理的对照小鼠更易感染。当在感染前1天、14天和21天给小鼠给予二氧化硅时，观察到对组织胞浆菌的易感性增加，但在3天和7天时未观察到。用亚致死剂量的组织胞浆菌攻击后存活30天的经二氧化硅处理的小鼠，其脾脏中的活菌数量比未处理对照小鼠的多23倍。注射二氧化硅后12小时，脾细胞对刀豆球蛋白A和植物血凝素的增殖反应未受影响，但在1至21天之间显著降低。相比之下，二氧化硅处理不影响脾细胞对脂多糖的增殖反应。二氧化硅颗粒在体外对小鼠腹腔巨噬细胞具有细胞毒性，但对淋巴细胞无细胞毒性。这些结果表明，巨噬细胞在对组织胞浆菌病的天然免疫中起重要作用。
