Cooke B A, Aldred L F, Hunter M G, Sullivan M H, Dix C J
J Steroid Biochem. 1983 Jul;19(1A):359-66.
The effect of the isolation and purification procedures used to obtain mouse and rat Leydig cells on their viability and steroidgenic properties has been investigated. It was found that mechanical dispersion of rat testes prior to collagenase dispersion had a deleterious effect on the steroidogenic capacity of the cells. Omission of the mechanical disruption and separation of the collagenase-dispersed cells on Percoll gradients yielded 3 bands of cells. Band III (density 1.070 g/ml) contained highly pure viable Leydig cells of high steroidogenic capacity. Diaphorase histochemistry (but not trypan blue exclusion) revealed that bands I and II contained high amounts of damaged Leydig cells. These results are discussed in relation to the previously reported heterogeneity of Leydig cells. Both mouse and rat Leydig cells (band III) in monolayer culture maintained their responsiveness to LH for several days. The rat (but not mouse) Leydig cells contained LHRH receptors and responded to LHRH in a biphasic manner: initially a stimulation of both basal and LH increased steroidogenesis occurred which after 24 h became inhibitory.
已对用于获取小鼠和大鼠睾丸间质细胞的分离纯化程序对其活力和类固醇生成特性的影响进行了研究。发现胶原酶分散前对大鼠睾丸进行机械分散对细胞的类固醇生成能力有有害影响。省略机械破碎以及在Percoll梯度上对胶原酶分散的细胞进行分离，得到了3条细胞带。带III（密度1.070 g/ml）包含具有高类固醇生成能力的高度纯的活睾丸间质细胞。黄递酶组织化学（而非台盼蓝排斥法）显示带I和带II含有大量受损的睾丸间质细胞。结合先前报道的睾丸间质细胞异质性对这些结果进行了讨论。单层培养的小鼠和大鼠睾丸间质细胞（带III）在数天内维持了对促黄体生成素（LH）的反应性。大鼠（而非小鼠）睾丸间质细胞含有促性腺激素释放激素（LHRH）受体，并以双相方式对LHRH作出反应：最初，基础和LH刺激的类固醇生成均增加，24小时后变为抑制作用。
