Bouillon R
J Steroid Biochem. 1983 Jul;19(1C):921-7. doi: 10.1016/0022-4731(83)90035-3.
Methods have been developed for the measurement of many, but not all, of the vitamin D metabolites. These methods are still laborious because of the necessity for extensive chromatographic purification but this may improve in the near future with the development of direct assays. By analogy with other steroid hormones, the production of better, and especially more specific, antibodies might be expected. In view of the numerous vitamin metabolites and isomers, chromatographic separation will however probably remain necessary for compounds present in low concentrations. The clinical usefulness of serum measurements of 25-OHD is now beyond doubt, especially since vitamin D deficiency is much more endemic than previously recognized. Measurement of 1,25-(OH)2D might also contribute to the clinical evaluation of disturbances of calcium metabolism but whether this also applies to other vitamin D metabolites awaits further research.
已开发出多种测量方法来测定许多（但并非全部）维生素D代谢物。由于需要进行广泛的色谱纯化，这些方法仍然很繁琐，但随着直接检测方法的发展，这种情况在不久的将来可能会得到改善。与其他类固醇激素类似，可以预期会产生更好、尤其是更特异的抗体。鉴于存在众多维生素代谢物和异构体，对于低浓度存在的化合物，色谱分离可能仍然是必要的。血清25-羟基维生素D测量的临床实用性现在毋庸置疑，尤其是因为维生素D缺乏比以前认识到的更为普遍。1,25-二羟基维生素D的测量也可能有助于钙代谢紊乱的临床评估，但这是否也适用于其他维生素D代谢物还有待进一步研究。
